| Registro completo |
| Provedor de dados: |
PAB
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| País: |
Brazil
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| Título: |
Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene
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| Autores: |
Curi,Rogério Abdallah
Chardulo,Luis Artur Loyola
Silveira,Antônio Carlos
Oliveira,Henrique Nunes de
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| Data: |
2008-05-01
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| Ano: |
2008
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| Palavras-chave: |
Calpastatin gene
Meat texture
PCR-RFLP
SNP
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| Resumo: |
The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500014
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| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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| Relação: |
10.1590/S0100-204X2008000500014
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| Formato: |
text/html
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| Fonte: |
Pesquisa Agropecuária Brasileira v.43 n.5 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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